Molecular Biology

StemCell Services offer advanced protein engineering capabilities from custom design, cloning & synthesis, developing stem cell systems for functional validation and custom readout assays, to the immortalisation of stem cell lines, our scientists have the knowledge and experience to achieve your specific goals.

  • RNA / DNA Extraction & Sequencing

    • Target sequence is obtained from Genbank
    • Primers are designed and sequence amplified by PCR

  • Cloning of Gene(s) of Interest

    • Clones are confirmed by DNA sequence analysis
    • Subcloned into mammalian vector using either:
      • Adenoviral, or
      • Lentiviral Systems

  • Transfection & Electroporation

    • Target gene insert is transfected into a human stem cell line using AMAXA Technology. This technology:
      • Enables the highest transfection efficiencies and protein expression levels in a wide variety of stem cells
      • Can be used to engineer stem cell lines to validate genes of interest or build specialist functional cell lines

  • Manipulation of Target Genes

    • Over-Expression
    • siRNA Knockdown

  • Recombinant Protein Production

  • Cell Line Production

    • Antibiotic selection of human cells expressing the target in either
      • Transient or
      • Stable Cells

  • Reporter Genes & Assays Systems

    • Expression of Reporter Genes to measure specific activity
    • Construction of Reporter Assay Systems for validation

  • Confirmation & Functional Validation

    • Confirmation by Real time PCR & Western blots
    • Functional validation e.g. test neuronal cell function either in our FLEXstation III or by traditional patch clamping to confirm neurons are functional

  • ADDITIONAL SERVICES

    • Routine testing of both viral and mycoplasma levels by PCR-based technology.